TABLE 6.
Differences in MCL PHA accumulation from unrelated carbon sources in recombinant E. coli strains
| Strain (genotype) | PHA content (%) [wt/wt]a | Monomer-supplying enzyme | Range of PHA composition (mol%)b
|
Source | |||
|---|---|---|---|---|---|---|---|
| 3HB (C4) | 3HHx (C6) | 3HO (C8) | 3HD (C10) | ||||
| JMU193 (fadR::Tn10 fadB64) | 0.1-2.0 | E. coli TesA | 0 | 21-33 | 62-71 | 3-8 | 15 |
| S17-1 (recA tra proA thi-1) | 1.5-3.3 | P. putida PhaGc | 0 | 0 | 0 | 100 | 25 |
| LS1298 (fadB) | 3.2 | U. californica Tes | 0 | 0 | 0 | 100 | 26 |
| RS3097 (fadR) | 3.4 | U. californica Tes | 0 | 0 | 0 | 100 | 26 |
| JM109 | 0.3-4.9 | E. coli FabHd | 74-99 | 1-27 | 1-6 | 1-2 | This study |
a
Range of PHA content accumulated by the strains in the particular study.
b
Range of mole percent PHA monomer composition achieved in the particular study.
c
PHA accumulation was done in the presence of the fatty acid biosynthesis inhibitor triclosan.
d
The modified FabH enzymes made in this study.