TABLE 1.
Primers used in this study
| Primer | Target | Sequence (degree of degeneracy) | Reference |
|---|---|---|---|
| 27f | 16S rRNA gene | AGAGTTTGATCMTGGCTCAG (2) | 28 |
| 1492r | 16S rRNA gene | TACGGYTACCTTGTTACGACTT (2) | 28 |
| int1.F | Integron integrases | GGGTCAAGGATCTGGATTTCG | 31 |
| int1.R | Integron integrases | ACATGCGTGTAAATCATCGTCG | 31 |
| intltdF | Integron integrase | CTNYTNTAYGGNWCNGG (2,048) | This study |
| intltdR | Integron integrase | TCYTGNACNGWNCKDATRTC (3,072) | This study |
| HS286 | Gene cassettes | GGGATCCTCSGCTKGARCGAMTTGTTAGVC (48) | 35 |
| HS287 | Gene cassettes | GGGATCCGCSGCTKANCTCVRRCGTTAGSC (384) | 49 |
| M13F | Cloned inserts | GTAAAACGACGGCCAG | Invitrogen, Promega |
| M13R | Cloned inserts | CAGGAAACAGCTATGAC | Invitrogen, Promega |
| T7 | Cloned inserts | TAATACGACTCACTATAGGG | Invitrogen, Promega |
| 534r | 16S rRNA gene | ATTACCGCGGCTGCTGG | 33 |
| 1100r | 16S rRNA gene | GGGTTGCGCTCGTTG | 28 |
| R1113 | 16S rRNA gene | GGGTTGCGCYCGTT (2) | This study |