TABLE 3.
Structures of gene cassettes from heavy-metal-contaminated mine tailingsa
| Cassette (sequence no.) | Space 1b (bp) | ORF (aa) | Space 2c (bp) | BLAST match (e value) | Organism | Comment |
|---|---|---|---|---|---|---|
| HMIC5 (1) | 24 | 82 | −4 | CAD18309 (e-19) | R. solanacearum | Associated with urease operon |
| HMIC7 (1) | 31 | 131 | −4 | AAK73292 (7e-23) | P. alcaligenes | SI cassette Ypar6 |
| HMIC8 (1) | 3 | 126 | −4 | None | ||
| HMIC9 (2) | 7 | 112 | 54 | None | ||
| HMIC10 (1) | 4 | 93 | 4 | None | ||
| HMIC11 (20) | 15 | 134 | 7 | NP_768178 (0.006) | B. japonicum | Unknown |
| HMIC12 (17) | 20 | 120 | 10 | None | ||
| HMIC15 (4) | 25 | 143 | −4 | NP_337685 (2e-9) | M. tuberculosis | Hydroxylaminobenzene mutase |
| HMIC16 (1) | 12 | 136 | 9 | NP_711470 (1e-11) | Leptospira interrogans serovar lai str | Unknown |
| HMIC18 (1) | 3 | 131 | −4 | None | ||
| HMIC20 (1) | 47 | 103 | 34 | None |
a
Gene cassette arrangement and significant BLAST matches. All gene cassettes had a TTAGGC 1R sequence and a GCCTAA 2R sequence.
b
Space between 1R sequence and start codon.
c
Space between stop codon and 1L sequence.