Long-range PCR amplicons and RFLP profiles of Tn1546-like elements from environmental VRE. Lanes M, phage λ digested with HindIII; lanes 1 to 4, long-range PCR amplicons of E. faecium 3 (lane 1), E. casseliflavus C2-W4 (lane 2), E. casseliflavus A2/1-S1 (lane 3), and E. faecium BM4147 (lane 4); lanes 5 to 8, RFLP profiles of the strains shown in lanes 1 to 4 following digestion with ClaI; lanes 9 to 12, RFLP profiles of the strains shown in lanes 1 to 4 following digestion with HpaI and BamHI.