TABLE 2.

Environmental VRE in which the structure and mobility of Tn1546-like elements were analyzed

Strain	Source	Species	PFGE type	Resistance patterna	Transfer of vancomycin resistance
					Approximate frequency	Plasmid detection
3b	Vancomycin sludge	E. faecium	A1	Van	1 × 10−4	Yes
69b	Vancomycin sludge	E. faecium	A2	Van	Not tested
174b	Vancomycin sludge	E. faecium	A3	Van	Not tested
WE3	Vancomycin sludge	E. faecium	A4	Van	Not tested
W5	Vancomycin sludge	E. faecium	B1	Van, Pen	2 × 10−5	Yes
W2	Vancomycin sludge	E. faecium	B2	Van, Pen	Not tested
LB 7	Activated sludge	E. faecium	B3	Van, Pen	Not tested
B2-S2	Raw sewage	E. faecium	B4	Van, Pen	Not tested
ØME1	Blue mussels	E. faecium	C	Van, Pen, Ery	1 × 10−4	Yesc
68b	Vancomycin sludge	E. mundtii	D1	Van, Ery	7 × 10−3	Yes
C2-W3	Vancomycin effluent	E. mundtii	D2	Van, Ery	Not tested
B3-W8	Raw sewage	E. mundtii	E	Van	8 × 10−4	Yesc
C1-S4	Polymyxin effluent	E. faecium	F	Van	No transfer
C1-S6	Polymyxin effluent	E. faecium	G	Van	3 × 10−5	Yes
C1-W5	Polymyxin effluent	E. durans	H	Van, Ery	2 × 10−3	Yes
C1-S2	Polymyxin effluent	E. casseliflavus	I1	Van, Pen	No transfer
C2-W4	Vancomycin effluent	E. casseliflavus	I2	Van	No transfer
A2/1-W1	Raw sewage	E. casseliflavus	I3	Van	Not tested
A2/1-S1	Raw sewage	E. casseliflavus	I4	Van	No transfer
B2-W5	Raw sewage	E. casseliflavus	I5	Van	No transfer
C2-W2	Vancomycin effluent	E. casseliflavus	K	Van	No transfer
LSIE1	Raw sewage	E. faecalis	J	Van, Pen	No transfer
LSIE6	Raw sewage	E. faecalis	L1	Van	5 × 10−6	No
A2/2-S1	Raw sewage	E. faecalis	L2	Van	1 × 10−7	No
E2-F	Nonagricultural soil	E. faecium	M	Van	6 × 10−5	Yesc
E4-F	Agricultural soil	E. faecium	N	Van	No transfer
C2-W1	Vancomycin effluent	E. durans	—d	Van	2 × 10−3	Yes
B2-S5	Raw sewage	E. mundtii	—d	Van, Ery	2 × 10−4	Yesc
E3-F	Agricultural soil	E. mundtii	—d	Van, Ery	3 × 10−4	No

^aVan, vancomycin; Ery, erythromycin; Pen, penicillin.

^bIsolated in a previous study (4).

^cThe plasmid was detected in the transconjugant but not in the donor strain.

^d—, not typeable by PFGE.