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. 2012 Apr 30;43(1):40. doi: 10.1186/1297-9716-43-40

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Copyright ©2012 Darpel et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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BTV viral proteins in tissue sections of selected sheep organs a 8 dpi with BTV-8.A1/A2: BTV core proteins (ORAB06- green) and NS2 (ORAB01- red) in the endothelium of a vessel in the prescapular lymph node at 8 dpi. Scale bar: 8 μm. B1/B2: BTV core protein (ORAB06- red) and NS2 (ORAB268) in the endothelial cells of a capillary in the skin at 8 dpi. Scale bar: 20 μm. C1/C2: BTV core proteins (ORAB06- red) and NS2 (ORAB268-green) in a gland branching off a hair in the skin at 8 dpi. Scale bar: 40 μm. The staining pattern of viral proteins in organs of infected sheep was comparable between animals infected with two different BTV strains, namely BTV2 and BTV 8, and between different antibodies to the same and/or additional viral proteins.