Table 1.
Anti-BTV antibodies used for tissue confocal microscopy
| Antigen | Antibody* | Species |
|---|---|---|
| Anti-NS2 (raised against bacterial expressed/purified NS2) |
ORAB01 |
polyclonal, rabbit (:1000) |
| Anti-NS2 (raised against bacterial expressed/purified NS2) |
ORAB53 |
polyclonal, rabbit (1:1600) |
| Anti-NS2 (based on NS2 mAb
[33] kindly provided by N.J.MacLachlan) |
ORAB268 |
mouse ascites (1:1000) |
| Anti-VP7 (raised against gel purified VP7) |
ORAB36 |
polyclonal, mouse (1:100) |
| Anti-Core (raised against purified BTV core particles) | ORAB06 | polyclonal, guinea pig (1:1000) |
*All antibodies are stored in the Orbi Virus Antibody Reference (ORAB) collection of the Vector-borne-viral Diseases Programme at IAH Pirbright.