Table 2.
Genotype determination by sequencing and POCPOW assay
| |
|
|
TqPCR |
|
|
|---|---|---|---|---|---|
| Gene | Type | Sample (n = 70) | POC (n = 44) | POW (n = 26) | % similarity |
|
SSU rRNA |
curtisi |
14 |
14 |
0 |
100 |
|
SSU rRNA |
wallikeri |
9 |
0 |
9 |
100 |
| LDH |
curtisi |
30 |
30 |
0 |
100 |
| LDH | wallikeri | 17 | 0 | 17 | 100 |
Samples were first identified by amplification of the SSU rRNA and ldh sequences, thanks to the primers displayed below. The results were compared with the TqPCR results and the similarity was calculated. Four P. o. curtisi samples and four P. o. wallikeri samples were sequenced for both SSU rRNA and ldh genes.