Table 4.
Basic characteristics of telomere lengths in humpback whales determined by different assays and quantitative methods
| T/S | SD | CV% | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
Assay |
Method |
N |
Av |
Min |
Max |
dMinMax |
Av |
Min |
Max |
Av |
Min |
Max |
SD/(dMinMax)% |
| I |
Pfaffl LR |
58 |
1.05 |
0.38 |
1.89 |
1.52 |
0.16 |
0.01 |
0.71 |
19.0 |
1.8 |
52.0 |
10.9 |
| |
Pfaffl RG |
60 |
1.29 |
0.38 |
2.42 |
2.04 |
0.22 |
0.01 |
0.47 |
15.8 |
1.6 |
47.8 |
11.0 |
| |
Ruijter |
60 |
1.36 |
0.36 |
2.60 |
2.24 |
0.25 |
0.02 |
0.81 |
17.1 |
4.7 |
41.2 |
11.2 |
| |
ddCq |
58 |
1.19 |
0.29 |
2.17 |
1.88 |
0.23 |
0.01 |
0.80 |
17.9 |
1.6 |
49.5 |
12.4 |
| II |
Pfaffl LR |
54 |
0.84 |
0.15 |
1.73 |
1.57 |
0.19 |
0.01 |
0.71 |
21.4 |
3.3 |
72.1 |
12.1 |
| |
Pfaffl RG |
49 |
0.77 |
0.15 |
1.80 |
1.65 |
0.31 |
0.03 |
1.86 |
35.5 |
6.0 |
131.0 |
19.1 |
| |
Ruijter |
51 |
0.73 |
0.15 |
1.51 |
1.36 |
0.16 |
0.01 |
0.58 |
21.8 |
3.3 |
72.1 |
11.9 |
| |
ddCq |
54 |
3.38 |
0.07 |
20.46 |
20.39 |
1.21 |
0.02 |
9.76 |
30.3 |
3.6 |
93.2 |
5.9 |
| III |
Pfaffl LR |
54 |
0.80 |
0.24 |
1.39 |
1.15 |
0.20 |
0.00 |
1.22 |
25.1 |
0.2 |
89.1 |
17.1 |
| |
Pfaffl RG |
55 |
0.87 |
0.19 |
1.62 |
1.43 |
0.31 |
0.04 |
1.12 |
49.6 |
4.5 |
372.4 |
21.6 |
| |
Ruijter |
56 |
0.82 |
0.17 |
1.40 |
1.23 |
0.21 |
0.01 |
1.25 |
26.4 |
0.6 |
89.1 |
17.3 |
| |
ddCq |
54 |
0.73 |
0.19 |
1.94 |
1.75 |
0.22 |
0.00 |
1.39 |
30.6 |
0.1 |
111.9 |
12.3 |
| IV |
Pfaffl LR |
59 |
1.58 |
0.44 |
2.87 |
2.43 |
0.14 |
0.01 |
0.42 |
8.7 |
1.3 |
29.6 |
5.7 |
| |
Pfaffl RG |
59 |
1.61 |
0.45 |
3.15 |
2.70 |
0.14 |
0.02 |
0.51 |
9.0 |
0.6 |
30.8 |
5.3 |
| |
Ruijter |
59 |
1.54 |
0.45 |
2.62 |
2.16 |
0.13 |
0.01 |
0.44 |
8.8 |
1.3 |
29.6 |
6.2 |
| ddCq | 60 | 1.53 | 0.45 | 2.93 | 2.48 | 0.12 | 0.01 | 0.31 | 8.2 | 1.3 | 27.2 | 4.9 |
N = number of samples after outlier removal; T/S = Telomere length; Av = average; Min = minimum T/S; Max = maximum T/S; dMinMax = difference between minimum and maximum T/S; SD = standard deviation of T/S estimates; CV% = the coefficient of variation of T/S estimates in percent; SD/(dMinMax) = the ratio in percent between SD and the difference between minimum and maximum T/S. Pfaffl LR = the Pfaffl method with baseline corrected in LinRegPCR; Ruijter = the Ruijter method; ddCq = the comparative Cq method; Pfaffl RG = the Pfaffl method with baseline corrected in Rotor-Gene or ABI software. See text for details.