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. 2012 Aug 28;11:297. doi: 10.1186/1475-2875-11-297

Figure 3.

Figure 3

The effect of staursoporine on Plasmodium berghei ookinetes. Ookinetes were incubated in ookinete medium only (control) or 15 μM staurosporine for 30 min (staursoporine treated), washed with phosphate buffered saline and assayed for the presence of condensed chromatin, (acridine orange assay), caspase-like activity (CaspaTag assay), and loss of mitochondrial membrane potential (JC-1 assay). Paired T-tests were performed to compare the level of apoptotic activity for control and treated samples within each assay. Error bars = SEM for three experimental repeats, n = 50-100 ookinetes per sample. Within assays, significant differences are indicated by different letters.