Table 1.
Comparison of BLV proviral detection by BLV-CoCoMo-qPCR, the TaqMan MGB assay, and TaKaRa cycleave PCR
|
pBLV-IF |
CoCoMo-qPCRa | Lew Methodb |
TaKaRa |
|---|---|---|---|
| (copy number) | Cycleave PCRc | ||
| 100 |
3/3d |
3/3 |
3/3 |
| 50 |
3/3 |
3/3 |
3/3 |
| 25 |
3/3 |
3/3 |
3/3 |
| 12.5 |
3/3 |
3/3 |
3/3 |
| 6.25 |
3/3 |
3/3 |
3/3 |
| 3.125 |
3/3 |
3/3 |
3/3 |
| 1.5625 |
3/3 |
0/3 |
3/3 |
| 0.78125 |
3/3 |
0/3 |
2/3 |
| 0 | 0/3 | 0/3 | 0/3 |
a BLV LTR genes were detected by BLV-CoCoMo-qPCR (Jimba et al., 2010).
b BLV pol gene was detected by TaqMan MGB assay developed by Lew et al. (Lew et al., 2004).
c BLV tax gene was detected by the cycleave PCR BLV detection kit (TaKaRa Bio inc.).
d Number detected per number tested.