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. 2012 Sep 21;8:167. doi: 10.1186/1746-6148-8-167

Table 2.

Comparison of BLV detection methods using 370 cattle

(a) Copy number/105 cells by BLV-CoCoMo-qPCR=0
Animal no. Copy numbera Nested PCR PHAb AGIDc ELISAd
1-39
 0
 -e
 -
 -
 -
40-53
 0
 -
 +f
 -
 -
54-85
 0
 -
 -
 -
 +
86
 0
 -
 -
 +
 -
87-123
 0
 -
 +
 -
 +
124-129
 0
 -
 -
 +
 +
130-138
 0
 -
 +
 +
 +
139-141
 0
 -
 -
 +
 NTg
142
 0
 -
 NT
 -
 -
143
 0
 -
 NT
 -
 +
144
 0
 -
 NT
 -
 NT
145-148
 0
 -
 +
 -
 NT
149,150
 0
 -
 +
 +
 NT
151,152
 0
 NT
 -
 -
 +
153
 0
 NT
 -
 +
 +
154
 0
 NT
 +
 -
 -
155
 0
 NT
 +
 +
 NT
156
 0
 NT
 +
 -
 NT
157-159
 0
 NT
 +
 -
 +
160,161
 0
 NT
 +
 +
 +
162
 0
 +
 -
 -
 +
163
 0
 +
 +
 -
 -
-
 163
 150
 85
 138
 57
+
 0
 2
 75
 25
 94
Tested
 163
 152
 160
 163
 151
(b) Copy number/105 cells by BLV-CoCoMo-qPCR>0
164
 1
 -
 +
 +
 +
165
 1
 +
 +
 -
 +
166
 1
 -
 -
 -
 -
167
 2
 +
 -
 +
 +
168
 3
 +
 +
 +
 +
169
 3
 NT
 +
 -
 +
170
 4
 +
 -
 +
 +
171
 5
 -
 +
 -
 -
172
 6
 +
 +
 +
 NT
173
 8
 +
 +
 -
 +
174
 9
 -
 +
 -
 +
175
 9
 +
 -
 -
 +
176
 9
 NT
 +
 +
 NT
177
 10
 +
 -
 +
 +
178
 12
 +
 +
 +
 +
179
 15
 +
 -
 +
 NT
180,181
 16,17
 +
 -
 +
 +
182
 17
 +
 +
 +
 +
183
 17
 NT
 +
 +
 +
184
 18
 -
 +
 +
 +
185
 19
 +
 -
 +
 +
186,187
 20,21
 -
 +
 +
 +
188
 22
 +
 -
 +
 +
189
 23
 -
 -
 -
 +
190
 25
 +
 +
 +
 +
191
 27
 -
 -
 +
 +
192
 33
 +
 -
 +
 +
193
 37
 -
 -
 +
 +
194
 39
 -
 +
 +
 +
195
 40
 -
 +
 +
 NT
196
 41
 +
 -
 +
 +
197
 42
 +
 +
 +
 +
198,199
 43,44
 +
 -
 +
 +
200
 45
 -
 +
 -
 +
201
 49
 +
 +
 +
 +
202
 50
 -
 +
 +
 +
203
 53
 +
 +
 +
 +
204
 55
 NT
 +
 +
 +
205
 58
 -
 +
 +
 +
206
 67
 +
 +
 +
 +
207
 68
 NT
 -
 +
 +
208–210
 71,74,79
 +
 +
 +
 +
211
 83
 -
 NT
 +
 +
212
 85
 +
 -
 +
 +
213
 91
 NT
 -
 +
 +
214,215
 92,96
 +
 +
 +
 NT
216
 99
 -
 -
 +
 +
217
 110
 +
 +
 -
 +
218
 110
 -
 -
 +
 +
219
 110
 +
 +
 +
 NT
220
 145
 +
 +
 -
 +
221
 145
 +
 +
 +
 +
222
 149
 -
 -
 +
 +
223
 154
 +
 NT
 +
 NT
224
 166
 +
 +
 +
 +
225
 166
 +
 -
 +
 NT
226
 176
 NT
 -
 +
 +
227
 176
 +
 +
 -
 +
228
 180
 -
 +
 +
 NT
229,230
 190,194
 +
 +
 +
 NT
231
 202
 NT
 +
 +
 +
232
 203
 +
 +
 +
 +
233
 231
 +
 -
 -
 +
234
 254
 +
 +
 +
 +
235
 261
 +
 +
 -
 +
236
 265
 +
 NT
 +
 +
237
 270
 +
 +
 +
 +
238
 295
 +
 -
 +
 +
239
 321
 +
 +
 +
 NT
240
 347
 +
 +
 +
 +
241
 349
 NT
 +
 +
 +
242,243
 363,366
 +
 +
 +
 +
244
 374
 +
 -
 +
 +
245
 472
 +
 +
 -
 +
246
 475
 +
 +
 +
 NT
247
 491
 +
 +
 -
 +
248
 565
 -
 -
 +
 +
249
 605
 +
 +
 +
 NT
250
 634
 -
 +
 +
 +
251,252
 664,684
 +
 -
 +
 +
253,254
 705,710
 +
 +
 +
 +
255-257
 835,868,875
 +
 -
 +
 +
258
 876
 +
 +
 +
 +
259
 942
 +
 -
 +
 NT
260,261
 1088,1170
 +
 -
 +
 +
262
 1196
 +
 +
 +
 NT
263
 1219
 +
 -
 +
 +
264,265
 1223,1225
 +
 +
 +
 +
266
 1233
 -
 -
 -
 NT
267,268
 1279,1282
 +
 -
 +
 +
269
 1445
 +
 NT
 +
 +
270
 1459
 +
 +
 +
 NT
271
 1495
 +
 -
 +
 +
272,273
 1501,1567
 +
 +
 +
 +
274
 1648
 +
 -
 +
 +
275
 1671
 +
 +
 +
 +
276
 1793
 +
 +
 +
 NT
277
 1844
 +
 -
 +
 NT
278
 1902
 +
 +
 +
 NT
279
 2068
 +
 -
 +
 +
280-282
 2130,2142, 2183
 +
 +
 +
 +
283
 2218
 -
 NT
 +
 +
284
 2219
 +
 -
 +
 +
285
 2326
 +
 NT
 +
 +
286
 2338
 +
 +
 +
 +
287
 2524
 +
 +
 -
 +
288
 2616
 +
 +
 +
 NT
289,290
 2621,2727
 +
 +
 +
 +
291
 2849
 NT
 +
 +
 +
292
 2855
 +
 -
 +
 +
293
 2963
 +
 +
 +
 +
294
 3062
 +
 -
 +
 +
295,296
 3097,3192
 +
 +
 +
 +
297
 3294
 +
 +
 +
 NT
298
 3512
 NT
 +
 +
 +
299
 3619
 NT
 +
 +
 NT
300
 3833
 +
 -
 +
 +
301
 3859
 NT
 -
 +
 NT
302
 3871
 +
 +
 +
 +
303
 3955
 +
 +
 +
 +
304
 4291
 +
 -
 +
 NT
305
 4517
 +
 +
 +
 +
306
 4623
 NT
 -
 +
 +
307,308
 4774,4837
 +
 +
 +
 +
309,310
 5079,5291
 +
 +
 +
 NT
311
 5296
 NT
 -
 -
 NT
312
 5453
 +
 +
 +
 NT
313
 5490
 +
 -
 +
 NT
314,315
 5551,5667
 +
 +
 -
 +
316
 5821
 +
 +
 +
 +
317,318
 6188,6423
 +
 -
 +
 NT
319
 6471
 NT
 +
 +
 +
320
 6567
 +
 +
 +
 +
321
 6686
 +
 -
 +
 +
322
 7127
 +
 +
 +
 +
323
 7303
 +
 -
 +
 NT
324
 7371
 +
 +
 -
 +
325
 7376
 +
 NT
 +
 +
326
 7665
 +
 -
 +
 +
327
 7835
 +
 +
 +
 NT
328
 7903
 +
 -
 +
 +
329
 8879
 +
 +
 +
 NT
330
 9048
 +
 -
 +
 +
331,332
 9275,9358
 +
 +
 +
 +
333
 9417
 +
 -
 +
 NT
334,335
 9843,10154
 +
 +
 +
 NT
336
 10252
 +
 +
 -
 +
337
 10363
 NT
 +
 +
 +
338
 11874
 +
 -
 -
 +
339
 12040
 NT
 -
 +
 NT
340
 12461
 +
 -
 +
 +
341
 12667
 +
 +
 +
 NT
342
 12791
 +
 -
 +
 +
343,344
 13136,13229
 +
 +
 +
 +
345
 13379
 +
 -
 +
 +
346
 13904
 +
 +
 +
 +
347
 14057
 +
 +
 +
 NT
348
 14433
 +
 +
 +
 +
349
 15602
 NT
 -
 +
 +
350
 15747
 +
 -
 +
 NT
351
 15982
 +
 NT
 +
 +
352
 16099
 +
 +
 +
 +
353
 16220
 NT
 +
 +
 +
354
 16984
 +
 +
 +
 +
355
 17577
 +
 +
 +
 NT
356
 18419
 +
 +
 +
 +
357
 18624
 NT
 +
 +
 NT
358
 19043
 NT
 +
 +
 +
359-361
 19732,21744 25912
 +
 +
 +
 +
362
 26883
 +
 -
 +
 NT
363
 27719
 NT
 +
 +
 NT
364
 28154
 +
 +
 +
 NT
365
 28934
 +
 +
 -
 +
366
 30098
 +
 -
 +
 NT
367
 32909
 +
 +
 -
 +
368,369
 36753,42015
 +
 +
 +
 NT
370
 52680
 NT
 -
 +
 NT
-
 0
 22
 72
 26
 2
+
 208
 161
 127
 181
 152
Tested 208 183 199 207 154

aCopy number/105cells by BLV-CoCoMo-qPCR.

bPHA, Passive hemagglutination antigen method performed with the Bovine Leucosis antibody assay kit “Nisseiken”.

cAGID, Agarose gel Immuno-diffusiontest was also performed for anti-BLV antibody detection with a commercial bovine leukemia virus antibody test kit.

dELISA, Enzyme-linked immunosorbent assay was performed with an ELISA kit for detecting anti-BLV antibody.

e-, Negative.

f+, Positive.

gNT, Not test.