Figure 1. LCI assay: Tested pairwise genes were fused to N-terminal domain of firefly luciferase (Nluc) or C-terminal domain of firefly luciferase (Cluc) and were cloned into T-DNA vector. Agrobacterium cultures harboring the tested pairwise plasmids were mixed at a 1:1 ratio and were co-infiltrated into N. benthamiana. 48 h later, leaves were detached and sprayed with 1 mM luciferin. Fluorescence chemical signal was captured with a low-light cooled CCD camera (Andor iXon CCD camera, Andor Technology Ltd.). Pictures were taken after 10 min explosure. (A). ERD2s interact with ARF1 and ARF1-GAP. “1” represents tested pairwised proteins ARF1-Cluc/GAP-Nluc; “2”: ERD2a-Cluc/ARF1-Nluc; “3”: ERD2a-Cluc/GAP-Nluc; “4”: ERD2a-Cluc/Nluc; “5”: ERD2b-Cluc/ARF1-Nluc; “6”: ERD2b-Cluc/GAP-Nluc; “7”: ERD2b-Cluc/Nluc; “4” and “7” are negative control; (B). ERD2a/2b oligomerize homologously or heterologously. “8” is negative control expressing Nluc and Cluc; “9”: ERD2a-Cluc/ERD2a-Nluc; “10”: ERD2a-Cluc/ERD2b-Nluc; “11”: ERD2b-Cluc/ERD2b-Nluc; “12”: ERD2a-Nluc/ERD2b-Cluc; “PC” is positive control expressing Cluc-AtRar1/AtSgt1a-Nluc (At: Arabidopsis).