Figure 1. Nitrogen starvation induces root elongation in an OsGLU3 dependant way in rice. (A) The phenotype of WT (SSBM) and Osglu3–1 under nitrogen-starvation. a, WT under control condition; b, WT under nitrogen starvation; c, Osglu3–1 under control condition; d, Osglu3–1 under nitrogen starvation. Bar = 2cm. (B) The phenotype of WT (Dongjin) and the T-DNA insertion mutant Osglu3–2 under nitrogen starvation. a, WT under control condition; b, WT under nitrogen starvation; c, Osglu3–2 under control condition; d, Osglu3–2 under nitrogen starvation. a-b, Bar = 2cm; c-d, Bar = 1cm. (C) Nitrogen-starvation could not induce root cell elongation in Osglu3–1. (D) The root crystalline cellulose (TFA-insoluble) content of Osglu3–1 was not obviously changed under nitrogen starvation. Data are mean values from three independent experiments, each performed in triplicate. Error bars represent standard error (SE). Different letters are used to indicate means that differ significantly (p < 0.05). CWM: cell wall material. (E) Nitrogen starvation could not induce the cell elongation in Osglu3–1.Bar = 50µm. (F) Restricted OsCYCB1;1:GUS expression in Osglu3–1 under nitrogen starvation. Bar = 100µm