Figure 3. Camalexin accumulation. Four week old plants (Col-0, mpk11, pad4–1) were inoculated with virulent Pma ES4326 (OD600 = 0.002) and harvested 24 and 48 h later. Camalexin was extracted as described previously6,21 and quantified by comparison with standards containing known amounts of camalexin. Two independent biological experiments each consisting of 8 biological replicates were performed (each biological replicate contained 3 leaf discs from 2 inoculated leaves from 1 plant). Data were combined using a mixed linear model and the statistical significances are indicated (n.s. = not significant, *** = p < 0.001).