Figure 1. In vitro conidial germination assay and transcriptional regulation of lipid droplet mobilization. (A) Conidia of M. oryzae wild-type strain P131 and an AGT1 knockout mutant (∆Moagt1) were incubated on an artificial inductive surface (plastic coverslips) and visualized under a light microscope for lipid droplet movement during conidial germination. Arrows indicate lipid droplets. Bars: 10 µM. (B) Conidia of M. oryzae strain P131 expressing pAGT1::eGFP (eGFP under the control of the AGT1 native promoter) were stained with Nile red and visualized under a confocal laser scanning microscope (Zeiss Confocor2–LSM 510). eGFP and Nile red were excited with an Argon laser (488 nm). Fluorescence signals were captured through the band-pass emission filter 505–530 nm for eGFP and with the long pass emission filter 650 nm for Nile red. Bar: 10 µM.