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. 2012 Nov 5;7(11):e49384. doi: 10.1371/journal.pone.0049384

Figure 3. Effect of (+)-SKF 10047 on NaV1.2 in the presence of G protein and PKC or PKA signaling pathway inhibitors.

Figure 3

A, Histograms of the means±SEM of the peak current inhibition percentages recorded from the NaV1.2-transfected HEK293T cells. The inhibitory effect of (+)-SKF 10047 on NaV1.2 was not altered by the presence of PKA inhibitors (H-89 and PKAi), PKC inhibitors (BIM I and Gö6976), a G protein agonist (GTPγS), a Gs antagonist (NF 449) or a Gi/o antagonist (NF 023) in the pipette solution. P>0.05, compared to SKF-1007 alone. B, Pre-incubating the HEK293T cells with CTX (a Gs activator) or PTX (a Gi/o inhibitor) for different lengths of time and at different concentrations did not alter the inhibitory effects of (+)-SKF 10047 on NaV1.2. (P>0.05, compared to the untreated cells).