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. 2012 May 30;12:207. doi: 10.1186/1471-2407-12-207

Table 1.

Primer sequences

Gene Symbol Gene Name Primer Sequences
Housekeeping genes
 
 
ACTB
actin, beta
F: 5'-AACTGGAACGGTGAAGGTGAC-3'
 
 
R: 5'-GACTTCCTGTAACAACGCATCTC-3'
HPRT1
hypoxanthine phosphoribosyltransferase 1
F: 5'-ACATCTGGAGTCCTATTGACATCG-3'
 
 
R: 5'-TTAAACAACAATCCGCCCAAAGG-3'
GAPDH
glyceraldehyde-3-phosphate dehydrogenase
F: 5'-GTGAAGGTCGGAGTCAACG-3'
 
 
R: 5'-GGTGAAGACGCCAGTGGACTC-3'
TBP
TATA box binding protein
F: 5'-GGTGTTGTGAGAAGATGGATGTTG-3'
 
 
R: 5'-CCAGATAGCAGCACGGTATGAG-3'
Target genes
 
 
CDKN1A
cyclin-dependent kinase inhibitor 1A (p21, Cip1)
F: 5'-ACTAGGCGGTTGAATGAGAGGTTC-3'
 
 
R: 5'-CAGGTCTGAGTGTCCAGGAAAGG-3'
EDN1
endothelin 1
F: 5'-CCAACCATCTTCACTGGCTTCC-3'
 
 
R: 5'-GTCAGACACAAACACTCCCTTAGG-3'
EEF1E1
eukaryotic translation elongation factor 1 epsilon 1
F: 5'-TGCGGGAGGTTCTTGTTCTG-3'
 
 
R: 5'-CTGTTAGACTTGGACCATTGTTTG-3'
GPR56
G protein-coupled receptor 56
F: 5'-CTACAGCCGAAGAATGTGACTC-3'
 
 
R: 5'-GCAGAAGCAGGATGTTTGGG-3'
MRE11A
MRE11 meiotic recombination 11 homolog A (S. cerevisiae)
F: 5'-GATGATGAAGTCCGTGAGGCTATG-3'
 
 
R: 5'-TGTTGGTTGCTGCTGAGATGC-3'
NFKB1
nuclear factor of kappa light polypeptide gene enhancer in B-cells 1
F: 5'-CCGTTGGGAATGGTGAGGTC-3'
 
 
R: 5'-TTGAGAATGAAGGTGGATGATTGC-3'
OBFC2B
oligonucleotide/oligosaccharide-binding fold containing 2B
F: 5'-GACGATGTTGGCAATCTG-3'
 
 
R: 5'-TGGCTCACTGAAGTTAGG-3'
PML
promyelocytic leukemia
F: 5'-CCAAGGCAGTCTCACCAC-3'
 
 
R: 5'-TTCGGCATCTGAGTCTTCC-3'
SOD2
superoxide dismutase 2, mitochondrial
F: 5'-GGTGTCCAAGGCTCAGGTTG-3'
    R: 5'-GTGCTCCCACACATCAATCCC-3'