Figure 3 .

Western blot analysis of brain from small ruminants and P2 Tg338 mice bioassay recipients. Western blot analysis of brain from goat 3538 (lane 1, 150 μg starting wet weight brain ), goat 3558 (lane 3, 250 μg ), goat 30–75 (lane 5, 250 μg) and sheep 3178 (lane 7, 350 μg), and brain from P2 mice from each of those 4 inoculum groups (lanes 2, 4 , 6 and 8, respectively, loaded with 60–100 μg brain) after digestion with proteinase K (50 μg/ml final concentration for goats and sheep, 100 μg/ml final concentration for mice) and detection with mAb F99/97.6.1, binding a carboxyl epitope (A) or mAb P4, binding an amino terminal epitope (B). Lane 9 contains brain (300 μg) from an age-matched uninoculated Tg338 mouse, homogenized and treated with proteinase K as described for tissue from P1 and P2 mice. Molecular mass markers are shown on the left.