Figure 3. Splenocytes were isolated from 4 tumor bearing, PBS (A, left panel) or Lm-LLO-E7 (B, left panel) treated mice and analyzed by flowcytometry for the presence of IL-17A⁺ cells. Vaccination induced increases in mean percentage of total γδ⁺ T cells and CD4⁺ cells were not statistically significant. Vaccination did not alter the percentages of CD4⁺, IL-17⁺ cells or CD4⁺, IL-17^- cells (B, right panel) relative to PBS treated control mice (A, right panel). γδ⁺ T cells, derived from gates in panels A and B (left panels), were further analyzed for the presence of IL-17 (C, D). Vaccination increased the percentages of γδ⁺, IL-17⁺ splenocytes in treated mice (D) relative to PBS treated mice (C). Data from individual mice are shown (C, D).