Figure 2. DCs do not migrate from the tumor site. Naïve and AB1-HA bearing mice were injected with 2x10⁷ fluoresbrite carboxy YG microbeads s.c. or i.t. respectively. One and three days later, tissues were harvested, stained with anti-CD11c Ab and analyzed by flow cytometry. A, Representative profiles of tumor, TDLN and normal LN DCs at days 1 and 3 post injection. Bead+ DCs gated as indicated. B, Number of tumor infiltrating bead+ DCs at days 1 and 3 post injection, Four mice per group. C, Number of bead+ DCs in the TDLN (ie. arriving from the s.c. tumor) vs. normal LN (i.e., arriving from tumor-free skin) at days 1 and 3 post injection. Four mice per group.