Figure 4. (A,B) H&E and LFB staining of the subventricular zone revealed the total absence of demyelination in the brains of immunized when compared with healthy brains. Highly myelinated areas appear densely stained with LFB (magnification 5X and 40X). (C,D) H&E staining of cerebellar tissue showed a structural integrity (magnification 5X) that was also confirmed by strongly positive GLAST staining at the level of Bergmann glial processes in immunized mice when compared with healthy mice (magnification 2.5X and 20X). Representative images from three mice are shown.