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. 2012 Nov 5;7(11):e49082. doi: 10.1371/journal.pone.0049082

Figure 3. Control of plasmid-free strains.

Figure 3

A. Comparison of NotI-PFGE patterns of plasmid containing strains (C9+, C10+) and isogenic plasmid-less derivatives (C9− and C10−). Red arrows indicate bands corresponding to plasmids in strains C9+ and C10+. B. Absence of plasmids in strains C9− and C10− was confirmed by multiplex PCR targeting a plasmid gene (ORF 20, 821-bp PCR product) and a chromosomal gene (mleA, 430-bp PCR product).