Table 2. Primers list.
| Primer name | Forward sequence (5′–3′) | Reverse sequence (5′–3′) | Target | Product (bp) |
| Detection of plasmid genes | ||||
| 8a/8b a | TAAGCAAACGGGGTCAACTC | TCAGGCCGAGGATCAATAAC | ORF 8 | 142 |
| oye1/oye2 | AGTAGTTATTCCGCCAATGA | ATGAATGGCTCCTTAGCATA | ORF 11 | 602 |
| oyeQ1/oyeQ2 a | TAAGGGATTTGAAGGCCAACT | TTGAAGAATTGCTTTAGCACCA | ORF 11 | 106 |
| repA1/repA2 | ATCGGCTCGAATATTCTCTCAA | CGTATTCTCTAGCCGCTTGTTT | ORF 15 | 911 |
| 2repA/NC1 | ATCACCTAGTAGACGAAGAG | GGTAGGCAGGTTCTAATC | ORF 15_ORF 1 | 6464 |
| orf20a/orf10 | AGTTAAGAACTATCGTAAGTCC | TTACTGGCCTCCTACTGAAC | ORF 20_ORF 10 | 9848 |
| repA2/NC3 | CGTATTCTCTAGCCGCTTGTTT | GCATTCGACTTTGCGGAATG | ORF 10_ORF 15 | 5199/8765b |
| oye1/orf13-2 | AGTAGTTATTCCGCCAATGA | TACAGCATACACTCACAGCA | ORF 11_ORF 13 | 2376/5942b |
| orf20a/orf20b | AGTTAAGAACTATCGTAAGTCC | AACAGGATCATAGTACATCAC | ORF20 | 821 |
| Detection of chromosomal genes | ||||
| OO1/OO2 | GTGCCGCTTTTTTGGATATTA | AGCAATTTTATCTTTATAGCT | mleA | 431 |
| 0812a/0812b | GATTATTACCAATTCGGCTG | ACGCCGGAAATAATGTAG | OEOE_0812 | 540 |
| rpoB1/rpoB2 a | ATGGAACGTGTTGTCCGCGA | GGATTGGTTTGATCCATGAA | rpoB | 148 |
a
Primers used in qPCR assays.
b
Product sizes obtained for pOENI-1 and pOENI-1v2.