Table 3. ORFs and predicted proteins of pOENI-1.

ORF	Position	%GC	Protein	Size (aa)	Predicted function	Best blast (organism, GenBank accession)	% identity
1	108-1619	44,0	TraI	503	DNA topoisomerase IA, TraI	L. pentosus, CCB84017	95
2	1742-1957	38,9	HP	71	Hypothetical protein	L. brevis, ZP_03940833	90
3	1961-3082	42,7	LtrC	373	LtrC-like protein	L. helveticus, ADX71206	96
4 a	3096-4053	36,9	Gdh	319	Glycerate dehydrogenase	Lc. kimchii, YP_003621246	70
5	4558-4172	42,1	HP	128	Hypothetical protein	P. claussenii, AEV96201	94
6	4871-4551	40,5	HP	106	Hypothetical protein	L. brevis, ZP_03940921	99
7	5865-5281	52,7	TetR	194	Transcriptional regulator, TetR	L.mali, ZP_09449471	99
8	6041-6787	53,3	TauE	248	Putative permease TauE	L. mali, ZP_09449472	99
9	7365-7952	41,8	Res	195	Resolvase	L. crispatus, YP_003601011	99
10	9124-8192	35,3	LysR	310	Transcriptional regulator, LysR	O. oeni, ZP_01543901	100
11	9267-10448	42,1	Oye	393	NADH: flavin oxidoreductase	O. oeni, ZP_01543900	100
12	11258-10575	45,5	Tnp	227	Transposase	O. oeni, ZP_01543898	100
13	11927-11649	41,6	ParB	92	Partition protein, ParB	L. casei, YP_794449	100
14	12831-11908	40,8	ParA	307	Partition protein, ParA	L. casei, YP_794448	99
15	13334-14449	39,5	RepA	371	Replication protein, RepA	P. claussenii, AEV96162	76
16	14709-14990	42,9	RelB	93	Addiction module antitoxin, RelB	L. hilgardii, ZP_03954201	100
17	14980-15486	35,9	PemK	168	Addiction module toxin, PemK	L. paracasei, ABA12818	99
18	15754-15476	34,8	HP	92	Hypothetical protein	L. brevis, YP_796419	97
19	15985-15776	30,0	HP	69	Hypothetical protein	L. mali, ZP_0944951	90
20 a	16256-18317	38,7	TraA	687	Putative nickase, TraA	L. pentosus, CCC15328	95

^apseudogenes, the characteristics of hypothetical full-length genes and proteins are provided.