Figure 3. PrP^Res levels in brains of GPI⁻ and WT mice infected with multiple mouse-adapted scrapie strains.

Normal brain homogenate as well as 22L, RML and ME7-infected brain homogenates were compared by immunoblotting. The sample brain equivalents were loaded into each lane. Lanes 1–2: WT and GPI⁻ NBH undiluted, respectively; Lanes 3–8: WT and GPI⁻ 22L BH undiluted and serially diluted 2-fold and 4-fold; Lanes 9–14: WT and GPI⁻ RML BH undiluted and serially diluted 2-fold and 4-fold; Lanes 15–20: WT and GPI⁻ ME7 BH undiluted and serially diluted 2-fold and 4-fold. A final concentration of 20 µg/mL PK was used to digest brain homogenates. Bands were detected with monoclonal antibody 6D11 as described in materials and methods.