Table 2.
Oligonucleotide primers used for site-directed mutagenesis and sequencing
| Primer | Positionc | Sequenced | Orientation |
|---|---|---|---|
| 154Fa |
3704-3740 |
5'-CCCTCGCACGCAGAGTGAACAACCGGCTGCCCACTTC-3' |
Sense |
| 154Ra |
3704-3740 |
5'-GAAGTGGGCAGCCGGTTGTTCACTCTGCGTGCGAGGG-3' |
Antisense |
| 72GFa |
2148-2181 |
5'-GTTTGACTGGACGCCGGGTTTGTCATTTGGACAC-3' |
Sense |
| 72GRa |
2148-2181 |
5'-GTGTCCAAATGACAAACCCGGCGTCCAGTCAAAC-3' |
Antisense |
| 72NFa |
2148-2181 |
5'-GTTTGACTGGACGCCGAATTTGTCATTTGGACAC-3' |
Sense |
| 72NRa |
2148-2181 |
5'-GTGTCCAAATGACAAATTCGGCGTCCAGTCAAAC-3' |
Antisense |
| 107Fa |
2253-2287 |
5'-GAGGAACGGGTGGGACATTGAGGTGACCGCTGTTG-3' |
Sense |
| 107Ra |
2253-2287 |
5'-CAACAGCGGTCACCTCAATGTCCCACCCGTTCCTC-3' |
Antisense |
| S1Fb |
207-227 |
5'-AACAGCTATGACCATGATTAC-3' |
Sense |
| S1Rb |
2677-2697 |
5'-GTTAGACACTTTCCCGTAGAC-3' |
Antisense |
| S2Rb | 4000-4019 | 5'-GACATGTCCTCCTGCATCTG-3' | Antisense |
a Primers for the construction of mutant viruses.
b Primers for sequencing.
c The position of primers was determined according to the sequence of pAsi.
d The mutated codons are bolded and underlined.