Table 1.
Amino acid sequence of C-terminal region of syn-wt and truncated or mutated mutants
| Syn-wt (ε0.1%280 nm = 0.354) | …GKNEEGAPQEGILEDMPVDPDNEAYEMPSEEGYQDYEPEA – 140 |
| Syn129 (0.227) | …GKNEEGAPQEGILEDMPVDPDNEAYEMPS – 129 |
| Syn118 (0.125) | …GKNEEGAPQEGILEDMPV – 118 |
| Syn103 (0.145) | …GKN – 103 |
| Syn130-140CF (0.356) | …GKNEEGAPQEGILEDMPVDPDNEAYEMPSNNGYQNYNPNA – 140 |
| Syn119-140CF (0.357) | …GKNEEGAPQEGILEDMPVNPNNNAYNMPSNNGYQNYNPNA – 140 |
| Syn130-140CF/Y136A (0.310) | …GKNEEGAPQEGILEDMPVDPDNEAYEMPSNNGYQNANPNA – 140 |
| Syn119-140CF/Y136A (0.310) | …GKNEEGAPQEGILEDMPVNPNNNAYNMPSNNGYQNANPNA – 140 |
| SynY125/133/136A (0.105) | …GKNEEGAPQEGILEDMPVDPDNEAAEMPSEEGAQDAEPEA – 140 |
| SynY125/133A (0.209) | …GKNEEGAPQEGILEDMPVDPDNEAAEMPSEEGAQDYEPEA – 140 |
| SynY125/136A (0.209) | …GKNEEGAPQEGILEDMPVDPDNEAAEMPSEEGYQDAEPEA – 140 |
| SynY133/136A (0.209) | …GKNEEGAPQEGILEDMPVDPDNEAAEMPSEEGAQDAEPEA – 140 |
| SynY125A (0.311) | …GKNEEGAPQEGILEDMPVDPDNEAAEMPSEEGYQDYEPEA – 140 |
| SynY133A (0.311) | …GKNEEGAPQEGILEDMPVDPDNEAYEMPSEEGAQDYEPEA – 140 |
| SynY136A (0.311) | …GKNEEGAPQEGILEDMPVDPDNEAYEMPSEEGYQDAEPEA – 140 |
| SynY136W (0.688) | …GKNEEGAPQEGILEDMPVDPDNEAYEMPSEEGYQDWEPEA – 140 |
| SynY136F (0.309) | …GKNEEGAPQEGILEDMPVDPDNEAYEMPSEEGYQDFEPEA – 140 |
| SynY136L (0.310) | …GKNEEGAPQEGILEDMPVDPDNEAYEMPSEEGYQDLEPEA – 140 |
| SynY136S (0.311) | …GKNEEGAPQEGILEDMPVDPDNEAYEMPSEEGYQDSEPEA – 140 |
| SynY136E (0.309) | …GKNEEGAPQEGILEDMPVDPDNEAYEMPSEEGYQDEEPEA – 140 |
Upper panel, The C-terminal amino acid sequence of Syn-wt and truncated or mutated mutants of acidic residues to Asn residue (marked by an underline). Syn130-140CF/Y136A and Syn119-140CF/Y136A are Tyr136 to Ala mutants of Syn130-140CF and Syn119-140CF, respectively. Middle panel, The C-terminal amino acid sequences of mutants, whose Tyr125, 133, 136 residue(s) were changed to Ala residue (marked by an underline). Lower panel, The C-terminal amino acid sequences of mutants, whose Tyr136 residue was changed to various different characteristic residues (marked by an underline).