Figure 1. PMT induces the covalent modification of Gα_q and Gα₁₁.

(A) Membrane proteins from Swiss 3T3 cells were separated by urea gel electrophoresis and Western blotted with anti-Gα_q/11 antibody. The locations of Gα_q and Gα₁₁ are indicated by arrows. Membrane proteins from Swiss 3T3 cells (B, D, F) left untreated or (C, E, G) treated with 150 pM PMT for 4 h were separated by 2-D gel electrophoresis and Western blotted with (B, C)anti-Gα_q/11, (D, E)anti-Gα_q or (F, G) anti-Gα₁₁ antibody. (H) Recombinant Gα_q subunit (lane 1) and membrane proteins from Swiss 3T3 cells (lane 2) were separated by SDS PAGE and Western blotted with anti-Gα_q (left panel) or -Gα₁₁ (right panel) antibody. Samples from at least 3 independent experiments were resolved with similar results.