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. 2012 Nov 5;7(11):e47188. doi: 10.1371/journal.pone.0047188

Figure 2. PMT induces the covalent modification of Gαi.

Figure 2

(A) Membrane proteins from Swiss 3T3 cells were separated by SDS PAGE and Western blotted with anti-Gαi-1, anti-Gαi-1-2 or anti-Gαi-1-3 antibody, as indicated. (B) A recombinant Gαi-1 subunit was analysed by SDS PAGE and Western blotted with anti-Gαi-1, -Gαi-1-2 or -Gαi-1-3 antibody, as indicated. Membrane proteins from Swiss 3T3 cells (C, E, G) left untreated or (D, F, H) treated with 150 pM PMT for 4 h were separated by 2-D gel electrophoresis and Western blotted with (C, D) anti-Gαi-1, (E, F)anti-Gαi-1-2 or(G, H) anti-Gαi-1-3 antibody. Samples from at least 3 independent experiments were resolved with similar results.