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. 2012 Nov 5;7(11):e47188. doi: 10.1371/journal.pone.0047188

Figure 5. Prolonged exposure of Swiss 3T3 cells to PMT causes the loss of Gαi but not Gq from cell membranes.

Figure 5

(A) Membrane proteins from Swiss 3T3 cells left untreated (lane 1) or treated with PMT at 1 nM for 16 h (lane 2) were separated by SDS PAGE and Western blotted with anti-Gαq/11 antibody. Membrane proteins from Swiss 3T3 cells (B) left untreated, or treated with 1 nM PMT for (C) 4 h or (D) 16 h were separated by 2-D gel electrophoresis and Western blotted with anti-Gαq/11 antibody. Samples from at least 3 independent experiments were resolved with similar results. Membrane proteins from Swiss 3T3 cells left untreated (lane 1) or treated with 1 nM PMT for 16 h (lane 2) were separated by SDS PAGE and Western blotted with (E) anti-Gαi-1-3 antibody or (F) an antibody recognising an internal epitope of Gαi-2. (G) Cytoplasmic proteins from Swiss 3T3 cells left untreated (lane 1) or treated with 1 nM PMT for 16 h (lane 2) were separated by SDS PAGE and Western blotted with anti-Gαi-1-3 antibody. Membrane proteins from Swiss 3T3 cells (H) left untreated, treated with 1 nM PMT for (I) 4 h or (J) 16 h were separated by 2-D PAGE and Western blotted with anti-Gαi-1-3 antibody. Samples from 3 independent experiments were resolved with similar results.