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. 2012 Nov 5;7(11):e47173. doi: 10.1371/journal.pone.0047173

Figure 3. Hippo target genes are affected in scrib mutant cells.

Figure 3

(a, e, g) Panels show diap1-lacZ (a), ex-lacZ (e), fj-lacZ (g) expression in wild-type wing imaginal discs. (b, f, h) scrib2/scrib3 mutant homozygous discs showing diap1-lacZ (b), ex-lacZ (f), fj-lacZ (h) expression. (c–d’’’) diap-lacZ levels in scrib mutant clones in wing imaginal discs from yw hs-Flp FRT82B scrib2/FRT82B ubi-GFP larvae (c). Note that a majority of the clones get eliminated 48h after induction in the wing pouch. (d–d’’’) Magnified view of the clone in the notum indicated by a yellow box. The clone (GFP negative) is smaller than its wild-type (2XGFP) twin-spot, and shows down-regulation of diap-lacZ (red in d’’ and greyscale in d’’’). The clone boundary is marked by yellow line (d,d”). The magnification and orientation of images in a–c, e–h is identical.