Figure 8. Effect of p53 silencing on the expression of BIM, NOXA and BAD.

HepG2 cells were transfected with 50 nM p53 siRNA (p53) or non-targeting control siRNA (NT) or left untransfected (/) for 24 hours. Minimum 6 hours later, cells were incubated under normoxia (N, 21% O₂) or hypoxia (H, 1% O₂) with (ETO) or without (CTL) etoposide (50 µM) for 16 hours. Proteins were detected in total cell extracts by western blotting, using specific antibodies. ß-actin was used as loading control. One experiment representative out of three. Uncropped western blots are presented in Figure S1.