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. Author manuscript; available in PMC: 2012 Nov 5.
Published in final edited form as: J Neurosci Res. 2009 May 15;87(7):1695–1702. doi: 10.1002/jnr.21971

Fig. 1. Immunostaining of KOPR with KT-2 antibody on cells and tissue sections.

Fig. 1

A) HEK cells were transiently transfected with FLAG-KOPR. Twenty four hours after transfection, cells were fixed and incubated with M2 monoclonal antibody and KT-2 together, followed by secondary antibodies AlexaFluor488-conjugated goat anti-mouse IgG (green) and TexasRed-conjugated goat anti-rabbit IgG (red), respectively. When the two images of transfected HEK cells taken from green and red channel were merged, most cells yielded yellowish color. Scale bar: 20 μm. B) Representative KOPR immunostaining with KT-2 antibody in rat spinal cord. Most dense immunoreactivity was found in the dorsal horn. Scale bar: 200 μm. White arrow heads: representative staining.