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. Author manuscript; available in PMC: 2012 Nov 5.
Published in final edited form as: Mol Pharmacol. 2008 Jan 22;73(4):1319–1330. doi: 10.1124/mol.107.042846

Fig. 4. Mutation of the hKOR ubiquitination sites did not change agonist-induced [35S]GTP γS binding and desensitization.

Fig. 4

CHO-FLAG-hKOR or CHO-FLAG-hKOR-10KR cells were incubated without or with 1 μM U50,488H for 30 min and washed. Membranes were prepared and U50,488H-stimulated [35S]GTPγS binding were determined as described in Materials and Methods. The EC50 and Emax values were showed in the table. Each value represents the mean ± s.e.m. of three independent experiments. * P<0.05, compared with the control group by two-tailed Student’s t test.