Figure 7. Peptide Retro D-p9 attenuate IgM and C3 deposition in response to I/R.
Prior to Sham or I/R treatment C57Bl/6 mice were treated with or without injection of 40 μM β2-GPI peptides, p9, D-p9 or Retro D-p9. Representative intestinal sections stained for IgM (A) or C3 (B) deposition. Additional sections were stained for myeloperoxidase (C). Fluorescent intensity was quantitated using Image J with threshold set on isotype control tissues. Each bar is the average ± SEM with 5–8 animals per treatment and 4–6 photographs per animal. Magnification for all photomicrographs and measurements were obtained at 200x. Microphotographs are representative of 3–4 animals stained in at least 3 independent experiments. * = p ≤ 0.05 compared to Sham + peptide, Φ = p ≤ 0.05 compared to I/R treatment animals not receiving peptides.