Table 1.
Genes that were up- or down-regulated in hAT2 cells by HSulf-1 or cadmium
| hAT2 cells | lacZ | HSulf-1 | ||||
|---|---|---|---|---|---|---|
|
| ||||||
| Gene | Description | Ctrl | Cd | Ctrl | Cd | Function |
| AKT1 | v-akt murine thymoma viral oncogene homolog 1 | 1 | −1.8 | −1 | −2.3 | Anti-apoptosis |
| BAG3 | Bcl-2-associated athanogene 3 | 1 | 4.5 | −1.1 | 2.2 | Anti-apoptosis |
| BCL2A1 | BCL2-related protein A1 | 1 | 2 | −2 | 2.8 | Anti-apoptosis |
| BIRC3 | Baculoviral IAP repeat containing 3 | 1 | 5 | 1.2 | 5.4 | Anti-apoptosis |
| BNIP1 | BCL2/adenovirus E1B 19kDa interacting protein 1 | 1 | 2 | 1.2 | 1.7 | Anti-apoptosis |
| IGF1R | Insulin-like growth factor 1 receptor | 1 | −1.7 | −2.3 | −2 | Anti-apoptosis |
| APAF1 | Apoptotic peptidase activating factor 1 | 1 | −2.7 | −1.8 | −1.5 | Pro-apoptosis |
| BCL2L11 | BCL2-like 11 (apoptosis facilitator) | 1 | 5.7 | 2.1 | 4.9 | Pro-apoptosis |
| BIK | BCL2-interacting killer | 1 | 3.3 | −1.3 | 3.8 | Pro-apoptosis |
| BNIP3 | BCL2/adenovirus E1B 19kDa interacting protein 3 | 1 | 2.2 | 1.3 | 2 | Pro-apoptosis |
| NOD1 | Nucleotide-binding oligomerization domain 1 | 1 | −4 | 1.1 | −3.4 | Pro-apoptosis |
| CARD8 | Caspase recruitment domain family, member 8 | 1 | −2.5 | −1.2 | −1.6 | Pro-apoptosis |
| CASP10 | Caspase 10 | 1 | −2.9 | −1.3 | −5.5 | Pro-apoptosis |
| CASP7 | Caspase 7 | 1 | 1.2 | 2.1 | 2.4 | Pro-apoptosis |
| CIDEA | Cell death-inducing DFFA-like effector a | 1 | 2.3 | −1.4 | 2.8 | Pro-apoptosis |
| DAPK1 | Death-associated protein kinase 1 | 1 | −3.6 | −4.2 | −3.8 | Pro-apoptosis |
| FAS | TNF receptor superfamily member 6 | 1 | −2.2 | 1.3 | −1.7 | Pro-apoptosis |
| HRK | Activator of apoptosis harakiri | 1 | 56.7 | 1.6 | 14.3 | Pro-apoptosis |
| LTA | Lymphotoxin alpha | 1 | 16.9 | 0.83 | 9.2 | Pro-apoptosis |
| TNFRSF21 | TNF receptor superfamily member 21 | 1 | −2.6 | −1.1 | −2.7 | Pro-apoptosis |
| TNFRSF9 | TNF receptor superfamily member 9 | 1 | 3.3 | 4.5 | 21.8 | Pro-apoptosis |
| TNFSF10 | TNF superfamily member 10 | 1 | −3.5 | 1.2 | −2.3 | Pro-apoptosis |
| CD40 | TNF receptor superfamily member 5 | 1 | −2 | −1.2 | −2.2 | Pro-proliferation |
| CD70 | TNF superfamily member 7 | 1 | 1.1 | −1.7 | 5.8 | Pro-proliferation |
| TNFSF8 | TNF superfamily member 8 | 1 | 2 | 1.7 | 3.4 | Pro-proliferation |
| BRAF | v-raf murine sarcoma viral oncogene homolog B1 | 1 | −1.7 | 1 | −2.7 | Unknown |
| CD27 | TNF receptor superfamily member 7 | 1 | 5.3 | 1.4 | 5.1 | Unknown |
| TP73 | Tumor protein P73 | 1 | 1.2 | −1.3 | 2.2 | Pr/An-apoptosis |
hAT2 cells were transduced for lacZ or HSulf-1 over-expression and treated with or without cadmium. Total mRNA of duplicate samples was isolated, Apoptosis PCR arrays were performed, and results were combined to analyze gene expression. Genes that were up- or down-regulated more than 2-fold over “lacZ control” levels are indicated. Red, up-regulation; Blue, down-regulation; Black, non-significant change.