Table 2.
Genes that were up- or down-regulated in H292 cells by HSulf-1 or cadmium
| H292 cells | lacZ | HSulf-1 | ||||
|---|---|---|---|---|---|---|
|
| ||||||
| Gene | Description | Ctrl | Cd | Ctrl | Cd | Function |
| AKT1 | v-akt murine thymoma viral oncogene homolog 1 | 1 | 1.2 | 2 | 2.4 | Anti-apoptosis |
| BAG1 | Bcl-2-associated athanogene 1 | 1 | −1.2 | −2.3 | −2 | Anti-apoptosis |
| BAG4 | Bcl-2-associated athanogene 4 | 1 | −1 | −2 | −1.3 | Anti-apoptosis |
| BCL2A1 | BCL2-related protein A1 | 1 | 1.2 | 5.3 | 5.4 | Anti-apoptosis |
| IGF1R | Insulin-like growth factor 1 receptor | 1 | −1.2 | −2.2 | −1.4 | Anti-apoptosis |
| APAF1 | Apoptotic peptidase activating factor 1 | 1 | 1.1 | 1.5 | 2.1 | Pro-apoptosis |
| BAX | BCL2-associated X protein | 1 | 1.2 | 2.1 | 2.9 | Pro-apoptosis |
| BIK | BCL2-interacting killer | 1 | −2 | 1.1 | 2.2 | Pro-apoptosis |
| BNIP3 | BCL2/adenovirus E1B 19kDa interacting protein 3 | 1 | 1.1 | 1.7 | 3 | Pro-apoptosis |
| CASP4 | Caspase 4 | 1 | 1.2 | 1.7 | 2.3 | Pro-apoptosis |
| CASP6 | Caspase 6 | 1 | −1.1 | −4.5 | −1.1 | Pro-apoptosis |
| CASP7 | Caspase 7 | 1 | 1 | 1.2 | 2.2 | Pro-apoptosis |
| CASP8 | Caspase 8 | 1 | −1.1 | −2.1 | 1.1 | Pro-apoptosis |
| CASP9 | Caspase 9 | 1 | −1.1 | −1.7 | −3.2 | Pro-apoptosis |
| CIDEA | Cell death-inducing DFFA-like effector a | 1 | −3.4 | 3.1 | 3.4 | Pro-apoptosis |
| DFFA | DNA fragmentation factor | 1 | 2.2 | 2.2 | 2.7 | Pro-apoptosis |
| FAS | TNF receptor superfamily member 6 | 1 | 1.1 | 2.3 | 3.6 | Pro-apoptosis |
| HRK | Activator of apoptosis harakiri | 1 | −1.2 | −1.9 | −2.1 | Pro-apoptosis |
| TNFRSF10A | TNF receptor superfamily member 10A | 1 | 1.2 | 1.7 | 2.4 | Pro-apoptosis |
| TNFRSF9 | TNF receptor superfamily member 9 | 1 | −1.3 | 7.3 | 7.7 | Pro-apoptosis |
| TNFSF10 | TNF superfamily member 10 | 1 | −1.6 | −2.8 | −3.5 | Pro-apoptosis |
| TP53 | Tumor protein 53 | 1 | −1.2 | −1.9 | −2.2 | Pro-apoptosis |
| GADD45A | Growth arrest and DNA-damage-inducible, alpha | 1 | 1.1 | 2 | 2.6 | Anti-proliferation |
| CD70 | TNF superfamily member 7 | 1 | 1.6 | 6.6 | 5.8 | Pro-proliferation |
| TNFSF8 | TNF superfamily member 8 | 1 | 1 | 5.3 | 6.2 | Pro-proliferation |
| CD27 | TNF receptor superfamily member 7 | 1 | −1 | 1.7 | 2.3 | Unknown |
| TRAF3 | TNF receptor associated factor 3 | 1 | 1.1 | 1.7 | 2.1 | Unknown |
| MCL1 | Myeloid cell leukemia sequence 1 | 1 | 1.3 | 2.1 | 2 | Pro/anti-apoptosis |
| TP73 | Tumor protein 73 | 1 | 1.1 | 2.5 | 3 | Pro/anti-apoptosis |
H292 cells were transduced for lacZ or HSulf-1 over-expression and treated with or without cadmium. Total mRNA of duplicate samples was isolated, Apoptosis PCR arrays were performed, and results were combined to analyze gene expression. Genes that were up- or down-regulated more than 2-fold over “lacZ control” levels are indicated. Red, up-regulation; Blue, down-regulation; Black, non-significant change.