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. Author manuscript; available in PMC: 2013 Nov 1.
Published in final edited form as: Plant J. 2012 Sep 25;72(4):537–546. doi: 10.1111/j.1365-313X.2012.05114.x

Figure 4.

Figure 4

PhyB must be able to bind to PIF3 to inhibit interaction with its target promoter. (A) Red light does not decrease the binding of PIF3 to its target promoter in the absence of phyB as determined by ChIP. The rDNA fragment was used as a non-binding control. The co-IPd DNA was expressed as relative values of PIF3-OX/phyB, Dc (SD, n=2, biological replicates). Dc indicates dark-grown PIF3-OX/phyB and Rc indicates red light-grown PIF3-OX/phyB. The upper panel indicates PIF3 protein levels after the ChIP (After IP). (B) N-terminal deleted PIF3 that cannot interact with phyB (PIF3ΔN) binds to its target promoter equally well both in Dc and Rc conditions as determined by ChIP. The co-IPd DNA was expressed as relative values of PIF3ΔN, Dc (SD, n=2, biological replicates).