Figure 3.
dsl-3 is required for commitment to differentiation in the AB lineage. (A) Proportion of embryos subjected to RNAi of the indicated gene that showed ectopic elt-2∷GFP expression. (B) Endogenous and ectopic elt-2∷GFP in terminal dsl-1(RNAi) and dsl-3(RNAi) embryos. (C) elt-2∷GFP in terminal P1-ablated wild-type and dsl-3(RNAi) embryos. Ablated blastomeres are denoted by dashed lines. (D) Proportion of isolated P1 or AB descendants of wild-type and dsl-3(RNAi) embryos expressing ectopic elt-2∷GFP. In all experiments, END-3 was induced by heat shock when the number of cells corresponded approximately to the 20E stage in intact wild-type embryos. Numbers in bars are the total number of embryos carrying the END-3 transgene. (*) Fisher's exact test, P < 0.01. Error bars represent standard error.