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. Author manuscript; available in PMC: 2013 Nov 15.
Published in final edited form as: J Immunol. 2012 Oct 5;189(10):4787–4796. doi: 10.4049/jimmunol.1200579

Figure 5. SAP dependence exhibited during HC-mediated selection of M3-restricted CD8+ T cells is intrinsic to M3-restricted thymocytes.

Figure 5

Mixture of BM cells from CD45.1 congenic B6 mice (SAP+CD45.1) with either SAP−/− (SAP−/−CD45.2) or B6 mice (SAP+CD45.2) were transferred into irradiated M3−/− (Figure 5A, B) or wildtype (Figure 5C) recipients. After 6 wk, BM chimeric mice were immunized with LemA-pulsed BMDC. Five days post-immunization, the M3/LemA-specific CD8+ T cells response was analyzed. Percentages of CD8+ M3/LemA-tetramer+ T cells isolated from the spleen and liver of immunized mixed BM chimeric mice are shown. Data represents 5–6 mice per group.