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. Author manuscript; available in PMC: 2013 Nov 1.
Published in final edited form as: Stem Cell Res. 2012 Jun 28;9(3):237–248. doi: 10.1016/j.scr.2012.06.003

Figure 2.

Figure 2

Characterization of hESCs under NCM conditions. (A) Karyotyping of WA01 cells cultured on MEFs for 35 passages (p35) and then grown under NCM conditions for 10 passages (i.e., mcp10). (B) Karyotype analysis of WA14 cells grown on MEFs for 30 passages (p30) and under NCM for 33 passages (mcp33). (C and D) Flow cytometric analysis of hESC marker expression in WA01 cells at mcp15 and WA01 cells grown on MEFs. (E) Two-way microarray analysis of BG01 cells grown as colonies and as NCM. Two-fold changes in mRNA levels (P<0.05) after normalization are shown. Partial up-regulated (red) and down-regulated (green) genes are indicated. (F) Hierarchical cluster analysis of mRNA expression in hESCs under NCM conditions and colony-type culture on MEFs.