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. Author manuscript; available in PMC: 2013 Nov 1.
Published in final edited form as: Stem Cell Res. 2012 Jun 28;9(3):237–248. doi: 10.1016/j.scr.2012.06.003

Figure 4.

Figure 4

Human ES cells under NCM culture maintain the potential to differentiate to multiple cellular fates in vitro. (A) Neuronal differentiation of WA01 cells: differentiated cells were examined with specific antibodies against MAP2 and β-tubulin III (Tuj1). The cells were co-stained with 4′,6-diamidino-2-phenylindole (DAPI). The images were merged. (B) Mesodermal differentiation of WA01 cells: differentiated cells were stained with specific antibodies against desmin and smooth muscle actin (SMA). (C) Endodermal and hepatic differentiation of WA01 cells: expression patterns of the hepatic markers α-fetoprotein (AFP), human albumin, and HNF4α by immunostaining the cells with specific antibodies.