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. Author manuscript; available in PMC: 2013 Nov 1.
Published in final edited form as: Stem Cell Res. 2012 Jun 28;9(3):237–248. doi: 10.1016/j.scr.2012.06.003

Table 1.

Non-colony type monolayer culture (NCM) of hESC and iPSC lines.a

Cell Resource pb mcpc Substrate Medium Inhibitord Karyotypee
BG01 BresaGenf 67 10 Matrigel (2.5%) mTeSR1 Y-27632 Normal
ES01 ESCI 74 9 Matrigel (2.5%) mTeSR1 Y-27632 Trisomy 20, 14%
ES04 ESCI 63 9 Matrigel (2.5%) mTeSR1 Y-27632 Normal
WA01 WiCell 35 10 Matrigel (2.5%) mTeSR1 Y-27632 Normal
WA07 WiCell 35 12 Matrigel (2.5%) mTeSR1 Y-27632 Normal
WA09 WiCell 33 11 Matrigel (2.5%) mTeSR1 Y-27632 Elevated polyploidy
WA09 WiCell 33 12 BD Purecoat/FN X-KSR/TeSR2 Y-27632 Elevated polyploidy
WA13 WiCell 27 10 Matrigel (2.5%) mTeSR1 Y-27632 Normal
WA14 WiCell 30 10 Matrigel (2.5%) mTeSR1 Y-27632 Normal
WA14 WiCell 30 11 Matrigel (2.5%) TeSR2 Y-27632 Normal
WA14 WiCell 30 30 Matrigel (2.5%) TeSR2 Y-27632 Normal
WA14 WiCell 30 11 BD Purecoat/FN X-KSR/TeSR2 Y-27632 Normal
WA14 WiCell 30 7 BD Purecoat/FN XGFS/TeSR2 Y-27632 NDg
TE03 Technion 59 6 Matrigel (2.5%) mTeSR1 Y-27632 Normal
UC06 UCSF 67 12 Matrigel (2.5%) mTeSR1 JAKi Normal
UC06 UCSF 67 7 Matrigel (2.5%) mTeSR1 Y-27632 Normal
SA01 CellArtis AB 22 3 BD Purecoat MEF-CM Y-27632 ND
BC1 Chen Gh 64 10 Matrigel (2.5%) mTeSR1 Y-27632 Normal
SCU-i10 NIH SCU ND 13 Matrigel (2.5%) mTeSR1 Y-27632 Normal

Notes and abbreviations:

a

All hESC cell lines described above were initially cultured on MEF-feeder layers as documented on the website of the NIH Stem Cell Unit (SCU). BG01 cells (p70) were adapted to NCM conditions in the presence of 50% MEF-conditioned medium and 50% regular hESC medium (containing 4 ng/ml FGF2). Additionally, 10 hESC cell lines were cultured under NCM conditions in mTesR medium (StemCell Technologies) or 2% XGFS/TeSR2 or 10% X-KSR/TeSR2 as indicated.

b

Passage numbers, designated for hESC lines grown on MEF-feeder layer.

c

Passage numbers, designated for the NCM culture of hESCs grown on Matrigel.

d

The chemical molecules, Y27632 (10 μM) and JAK inhibitor I (JAKi, 1 μM), were used for enhancing single-cell plating efficiency within the initial 24 h after plating the cells. The inhibitors were then removed and cells were grown in the media described above.

e

Karyotype: elevated percentages of polyploidy were found in WA09 (H9) cells.

f

Abbreviations are: BresaGen: BresaGen, Inc., Athens, GA; ESCI, ES Cell International; FN, fibronectin; Technion: Technion-Israel Institute of Technology, Haifa, Israel; UCSF: University of California, San Francisco, CA; and WiCell: Wisconsin Alumni Research Foundation (WARF) (WiCell Research Institute), Madison, WI.

g

ND, not determined.

h

Provided by Dr. Guokai Chen (NIH).