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. 2012 Aug 3;44(10):603–614. doi: 10.3858/emm.2012.44.10.069

Figure 4.

Figure 4

FTY720 induces AMPK phosphorylation in vivo as well as in vitro. (A) Western blot analysis for p-AMPK (Thr 172) protein in WAT of standard diet (n = 4, SD), high fat-diet (n = 5, HFD CON) and FTY720-treated obese mice (n = 5, HFD FTY720). (B) Levels of p-AMPK (Thr 172) were normalized to α-actin. The experiments were repeatedly performed to confirm the results. The data were analyzed using student's t-test. **P < 0.01, significant differences between SD and each treatment group; ##P < 0.01, significant differences between HFD-CON and HFD-FTY720 group (C) Preadipocytes were induced to differentiate with or without FTY-p 0.1, 1 and 10 µM for 4, 12 and 24 h. At indicated hours, the protein levels of p-AMPK were analyzed by Western blot. The experiments were repeatedly performed to confirm the results. (D) Level of p-AMPK was normalized to α-actin. The experiments were repeatedly performed to confirm the results. The data were analyzed using Student's t-test. **P < 0.01, significant differences between control and each treatment group.