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. 2012 Aug 3;44(10):603–614. doi: 10.3858/emm.2012.44.10.069

Figure 5.

Figure 5

FTY720 up-regulates ATGL, HSL, perilipin gene expression as well as activation of HSL. (A-C) Relative mRNA levels were measured by RT-qPCR for the ATGL (A), HSL (B) and perilipin (C) genes from WAT of SD, HFD-CON and HFD-FTY mice. (D) Western blot analysis for the phosphorylation status of Ser 563 of HSL in WAT of SD (n = 4, SD), high fat-diet (n = 5, HFD CON) and FTY720-treated obese mice (n = 5, HFD FTY720). (E) Levels of p-HSL (Ser 563) was normalized to α-actin using densitometry. The experiments were repeatedly performed to confirm the results. The data were analyzed using student's t-test. **P < 0.01, significant differences between SD and each treatment group; ##P < 0.01, significant differences between HFD-CON and HFD-FTY720 group.