Figure A2.

FM 1-43 and serotonin co-localization is not caused by cross-talk between fluorescence channels. FM 1-43 (blue green) and Serotonin (5-HT, magenta) images of the same cultured serotonergic neuron. In the merged image (FM 1-43, 14min and 5-HT, 21 min) white arrows show co-localized vesicular structures (recycled vesicles), yellow arrows mark spots visible only in the FM 1-43 channel, and red arrows mark spots visible only in the serotonin channel. FM 1-43 images recorded before (14 min) and after (35 min) serotonin imaging shows the same bright spots (by yellow arrows), but these are absent in the serotonin channel (5-HT, 21 min). Top left corner of each image shows the starting time of image recording following sham treatment (Intensities false are color coded).