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. 2012 Oct 10;32(41):14165–14177. doi: 10.1523/JNEUROSCI.1402-12.2012

Figure 3.

Figure 3.

Potentiation of synaptic transmission in the BLA–CeL projections by PACAP implicates postsynaptic expression mechanisms. A, Summary graphs demonstrating the time course of EPSC (filled symbols; n = 12) and PPR (open symbols; n = 12) magnitude changes produced by PACAP38 application (red horizontal bar). The EPSCs were recorded at −70 mV in voltage-clamp mode. The insets show averaged paired BLA–CeL EPSCs (50 ms interpulse interval) obtained before (black trace) and after (red trace) PACAP38 application. B, Summary of EPSC amplitude changes induced by PACAP38 for the experiments shown in A (n = 12). C, Summary of PPR magnitude changes for the experiments shown in A (n = 12). D, Examples of EPSCs (average of 10–15 traces) recorded at −70 mV or +50 mV before (black traces) and after PACAP38 application (red traces) in the presence of PTX (50 μm). The amplitude of AMPA receptor-mediated EPSC (EPSCAMPAR) was determined at −70 mV (at a black dot), while the amplitude of NMDA receptor EPSC (EPSCNMDAR) was determined at +50 mV and measured 50 ms after the stimulus (at a vertical dotted line). E, Summary plot demonstrating the time course of EPSCAMPAR magnitude changes in CeL neurons affected by PACAP38 application (red horizontal bar; n = 9). F, Values of the EPSCAMPAR/EPSCNMDAR ratio calculated before and after application of PACAP38 (n = 9) for the same recordings as in E. G, Examples of NMDAR EPSCs recorded in CeL neurons at +30 mV before (black trace) and after (red trace) PACAP38 application. Recordings were performed in the presence of PTX (50 μm) and NBQX (10 μm). H, Time course of EPSCNMDAR amplitude changes in response to PACAP application (red horizontal bar; n = 7). I, The EPSCNMDAR amplitude values before and after PACAP38 application (n = 7) for the same recordings as in H. Error bars indicate SEM.