| Stock Basal media | 500 ml |
| 1:1 DMEM:F12 | 480 ml |
| N2 supplement | 5 ml |
| 1M HEPES | 5 ml |
| Glucose | 3.0 g |
| N-acetylcysteine (60 μg/ml) | 1 ml |
| Neural survival factor-1 (NSF-1) | 10 ml |
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