| Component | Volume (1 reaction) | Volume ( 48 reaction) |
| 2x Single Cell RT reaction Buffer | 0.50 μl | 30.0 μl |
| RNase Inhibitor (10 U/μl) | 0.02 μl | 1.2 μl |
| 5X Single Cell RT enhancer | 0.15 μl | 9.00 μl |
| Single Cell RT Enzyme Mix | 0.04 μl | 2.4 μl |
| Advablue (OAX04227) | 0.1 μl | 6.00 μl |
| Nuclease free water | make up to 1 μl | make up to 60 μl |
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